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KMID : 0861020200350030001
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Korea Journal of Herbology
2020 Volume.35 No. 3 p.1 ~ p.8
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The effects of a Mixture of Puerariae Flos Extract and Citri Unshius Pericarpium Extract on Estrogenic Activities and Osteoclastogenesis
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Cho Ho-Song
Lee Bo-Young
Lee Won-Kyung
Lee Jun-Ho
Park Dong-Jun
Choi Chang-Il
Jin Mu-Hyun
Roh Seok-Seon
Ju Young-Sung
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Abstract
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Objectives : In this study, we examined the estrogenic activities and anti?osteo clastogenesis effects of PCE17, a mixture of PE (an extract of Puerariae Flos), and CE (an extract of Citri Unshius Pericarpium).
Methods : The estrogenic effect of PCE17, PE and CE were examined by ER-β/ERE reporter gene assay and proliferation assay in 293 T and MCF-7 cells. The expression of estrogen-responsive gene and protein were checked by Real Time-PCR (RT-PCR) and Western blotting in MCF-7 cells. Inhibitory effect of PCE17, PE and CE on RANKL-induced osteoclast differentiation were evaluated by TRAP staining and RT-PCR in primary osteoclast precursors from rat bone marrow cells.
Results : PCE17 and PE bind to ERs (estrogen receptors) and show estrogenic activities in 293T cells. They also stimulated the proliferation of MCF-7 cells and increased the expression of ER response gene, pS2. Tectorigenin, an active ingredient of PE, shows similar estrogenic activities in MCF-7 cells. PCE17 and CE inhibited RANKL-induced osteoclastogenesis in rat primary osteoclast precursor cells and down-regulated the osteoclast-specific genes of Nfatc1, Ctsk, and Acp5.
Conclusions : In conclusion, PCE17 may have therapeutic potential in cases of menopause and osteoporosis.
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KEYWORD
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PCE17, Puerariae Flos, Citri Unshius Pericarpium, Estrogenic Activities, Anti-osteoclastogenesis
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